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KMID : 1020220210210050555
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Journal of Korean Society of Dental Hygiene
2021 Volume.21 No. 5 p.555 ~ p.565
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Quantitative detection of peri-implantitis bacteria using real-time PCR
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Kim Min-Jung
Han Gyeong-Soon
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Abstract
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Objectives: This study was conducted to analyze peri-implantitis bacteria and identify their associations with health status and health activities.
Methods: Gingival sulcus fluid at the implant’s periodontal pockets sampled from the participants were analyzed by multiplex real time PCR.
Results: Participants had strains in the order of 100% F. nucleatum, 98.0% E. corrodens, and 96.0% P. micra, and the correlation between C. rectus and E. nodatum was high (p<0.01). Diabetic group (P. gingivalis, P.
nigrescens) hypertension (P. nigrescens), group with four or more periodontal pockets (P. gingivalis, T. dentica, P. intermedia, E. nodatum, and C. rectum), smoking (P. micra, E. corrodens), drinking (T. dentola), and scaling groups (C. rectus) were found to have more strains (p<0.05).
Conclusions: Representative pathogenic microorganisms detected in periodontal pockets of implants were similar to dental periodontal pockets; however there were differences in the amount and distribution of microorganisms, and they were affected by health status and health behavior.
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KEYWORD
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Multiplex real-time PCR, Oral pathogenic microorganisms, Peri-implantitis
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